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AP Biology — Unit 2 — Cell Structure & Compartmentalization — Drill 5

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About This Drill

AP Biology — Unit 2 — Cell Structure & Compartmentalization — Drill 5 is a practice drill. It contains 5 original questions created by Brian Stewart, a Barron's test prep author with over 20 years of tutoring experience.

Practice identifying organelle functions and the importance of cellular compartmentalization in eukaryotic cells with this AP Biology drill.

Passage

Eukaryotic cells maintain distinct internal compartments, each enclosed by membranes that create specialized microenvironments. This compartmentalization allows incompatible chemical reactions to occur simultaneously within a single cell. The endomembrane system -- which includes the endoplasmic reticulum (ER), Golgi apparatus, lysosomes, and vesicles -- coordinates the synthesis, modification, and transport of proteins and lipids. The rough ER is studded with ribosomes and is the primary site of synthesis for proteins destined for secretion or insertion into membranes. These proteins are threaded into the ER lumen as they are synthesized. They then travel via vesicles to the Golgi apparatus, where they undergo further modification (such as additional glycosylation) before being sorted and shipped to their final destinations. The smooth ER, which lacks ribosomes, is involved in lipid synthesis and detoxification reactions. Scenario: A researcher treats cells with brefeldin A, a compound that disrupts vesicle formation from the ER and blocks ER-to-Golgi transport. After treatment, the researcher observes that partially glycosylated proteins accumulate inside the rough ER and are absent from the cell surface.

Questions in This Drill

  1. The researcher observes that partially glycosylated proteins accumulate in the rough ER after brefeldin A treatment. Which of the following best explains this observation in terms of intracellular transport mechanisms?
  2. The passage describes how the endomembrane system maintains distinct compartments with specialized conditions. A student argues that cells could carry out all reactions in a single aqueous compartment without loss of function. Which of the following aspects of the endomembrane system described in the passage best refutes this claim?
  3. The smooth ER is particularly abundant in liver cells. Which of the following best explains this observation?
  4. A researcher inhibits the SNARE proteins required for vesicle fusion at the Golgi. Which of the following steps in the secretory pathway is directly disrupted?
  5. A mutation eliminates the signal sequence on a protein normally destined for the ER lumen. Which of the following is the most likely outcome?